other:Article Title: Antibody-mediated clearance of an ER-resident aggregate that causes glaucoma
Article Snippet: Antibody Identification Epitope & Clonality Experiment & dilution Mouse anti-c-myc 9E10 BioXCell Cat# BE0238 RRID: AB_2687720 Human c-myc (monoclonal) Panning (2 μg/ml) Mouse anti-M13 RLph1 HRP Santa Cruz Cat# sc-53004 RRID: AB_673750 anti-g8p (monoclonal) ELISA (1:2000) Goat anti-human IgG Fc HRP Southern Biotech Cat# 2048-05 RRID: AB_2795688 Human IgG Fc region (polyclonal) ELISA (1:2000) Goat anti-human kappa∗HRP Southern Biotech Cat# 2060-05 RRID: AB_619883 Human kappa light chain (polyclonal) ELISA (1:2000) Immunoblotting of purified proteins (1:5000) Streptavidin-HRP BC Biosciences Cat# 554066 n/a Western blot (1:2000) Commercial rabbit antimyocilin (ab41552) Abcam Cat# ab41552 RRID: AB_776605 Myocilin far N-terminal within residues 25-46 conjugated to KLH (polyclonal) Immunoprecipitation (1:1000) Fluorescent microscopy (1:500) Goat anti-rabbit∗HRP Avantor Cat# 95058-730 RRID: AB_2934079 Rabbit IgG (polyclonal) Immunoprecipitation (1:1000) Commercial mouse anti-myocilin antibody R&D Systems Cat# MAB3446 RRID: AB_2148649 Human myocilin LZ within residues 112–185 (monoclonal) Immunoblotting of cell culture samples (1:1000) Rabbit anti-β-actin Cell Signaling Technology Cat# 4970 RRID: AB_2223172 Human β-actin (monoclonal) Immunoblotting of cell culture samples (1:1000) Goat anti-mouse Starbright Blue 520 Bio-Rad Cat# 12005866 RRID: AB_2934034 Mouse IgG (polyclonal) Immunoblotting of cell culture samples (1:2500) Goat anti-rabbit Starbright Blue 700 Bio-Rad Cat# 12004161 RRID: AB_2721073 Rabbit IgG (polyclonal) Immunoblotting of cell culture samples (1:2500) Rabbit anti-calnexin Invitrogen Cat# PA5-34754 RRID: AB_2552106 Calnexin (polyclonal) Confocal microscopy (1:200) Mouse anti-FLAG Cell Signaling Cat# 8146 RRID: AB_10950495 DYKDDDDK Tag (9A3) (monoclonal) Confocal microscopy (1:200) Goat anti-rabbit Alexa Fluor 488 Thermo Fisher Cat# A-11034 RRID: AB_2576217 Rabbit IgG (polyclonal) Confocal microscopy (1:1000) Goat anti-mouse Cyanine5 Thermo Fisher Cat# A48255 RRID: AB_2534033 Mouse IgG (polyclonal) Confocal microscopy (1:1000) Rat anti-FLAG Alexa Fluor 488 Invitrogen Cat# MA1-142-A488 RRID: AB_2610653 Rat IgG (monoclonal) Fluorescent microscopy (1:500) Mouse anti-FLAG Sigma Cat# F3165-1MG RRID: AB_259529 Mouse IgG (monoclonal) Fluorescent microscopy (1:500) Fig. S1.
Virus:Article Title: Lipocalin 2 orchestrates resistance to ferroptosis via AXL.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 Cell Signaling Technology Cat #52455S; RRID:AB_2924984 Rabbit anti-pAXL (Y702) ThermoFisher Cat #PA5-64862; RRID:AB_2662770 Rabbit anti-AXL (C terminus) LS Bio Cat # LS-B12012-100 hFAB TM Rhodamine Anti-Actin Bio-Rad Cat #120041163 Goat anti-rabbit StarBright Blue 700 Bio-Rad Cat #12004161; RRID:AB_2721073 Rabbit anti-BCL-XL Cell Signaling Technology Cat #2764T; RRID:AB_2228008 Rabbit anti-BIM Cell Signaling Technology Cat #2819S; RRID:AB_10692515 Rabbit anti-BAK Cell Signaling Technology Cat #3814S; RRID:AB_2290287 Rabbit anti-lipocalin-2 antibody Abcam Cat #ab63929; RRID:AB_1140965 Chicken anti-GFP Aves Labs Cat #GFP-1020; RRID:AB_10000240 Mouse anti-Beta III Tubulin Promega Cat #G7121; RRID:AB_430874 Rabbit anti-Phosphorylated Histone H3 Cell Signaling Technology Cat #4499; RRID:AB_10544537 Rabbit anti-Cleaved Caspase 3 Cell Signaling Technology Cat #9661; RRID:AB_2341188 Bacterial and virus strains psPAX2 (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12260; RRID:Addgene_12260 pMD2.G (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12259; RRID:Addgene_12259 MISSION pLKO.1-puro Non-Mammalian shRNA Control Sigma TRC #SHC002 Lcn2 shRNA KD1 Sigma TRC #TRCN0000055328 Lcn2 shRNA KD2 Sigma TRC #TRCN0000055331 Lcn2 shRNA KD3 Sigma TRC #TRCN0000055332 Biological samples Human glioblastoma patient tissue This paper, Cleveland Clinic See STAR Methods Human non-tumor patient tissue This paper, Cleveland Clinic See STAR Methods Primary mouse astrocytes Silver et al. 80 See STAR Methods Chemicals, peptides, and recombinant proteins RPMI 1640 medium Cleveland Clinic Media Preparation Core N/A Fetal bovine serum Cleveland Clinic Media Preparation Core N/A Penicillin-Streptomycin Cleveland Clinic Media Preparation Core N/A Trypsin-EDTA Cleveland Clinic Media Preparation Core N/A Trypan Blue ThermoFisher Cat #15250061 Image-It Lipid Peroxidation Kit Invitrogen Cat #C10445 CellRox Deep Red ThermoFisher Cat #C10422 Deferoxamine (DFO) Sigma Cat #D9533-1G RSL3 (GPX4 inhibitor) SelleckChem Cat #S8155 Erastin SelleckChem Cat #S7242 Ferrostatin-1 (Fer-1) SelleckChem Cat #S7243 Liproxstatin-1 (Lip-1) SelleckChem Cat #S7699 Necrostatin-1 SelleckChem Cat #ab141053 Z-VAD-FMK InvivoGen Cat #tlrl-vad PAC-1 SelleckChem Cat #S2738 Bemcentinib (R428, BGB324) SelleckChem Cat #S2841 1x NP-40 lysis buffer ThermoFisher Cat #J60766.AP Protease inhibitor cocktail Sigma-Aldrich Cat #P8340 (Continued on next page) 16 Cell Reports 45, 116965, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor cocktail Sigma-Aldrich Cat #P5725 PMSF Sigma-Aldrich Cat #10837091001 Sodium acetate ThermoFisher Cat #A13184-30 Thioglycolic acid Fisher Scientific Cat #M0052252G Chromogenic solution (FerroZine) MedChemExpress Cat #HY-137805 FITC-annexin V BioLegend Cat #640914 DRAQ7 ThermoFisher Cat #D15105 Critical commercial assays CellTiter Glo Cell Viability Assay Promega Cat #G7573 RNeasy Mini Kit Qiagen Cat #74106 qScript cDNA SuperMix Quanta Biosciences Cat #95048-100 Fast SYBR Green Mastermix ThermoFisher Cat #4385618 Proteome Profiler Mouse Phospho-RTK Array Kit R&D Systems Cat#ARY014 Experimental models: Cell lines OL61 (mouse glioma) Castro-Lowenstein Laboratory, Univ.
Plasmid Preparation:Article Title: Lipocalin 2 orchestrates resistance to ferroptosis via AXL.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 Cell Signaling Technology Cat #52455S; RRID:AB_2924984 Rabbit anti-pAXL (Y702) ThermoFisher Cat #PA5-64862; RRID:AB_2662770 Rabbit anti-AXL (C terminus) LS Bio Cat # LS-B12012-100 hFAB TM Rhodamine Anti-Actin Bio-Rad Cat #120041163 Goat anti-rabbit StarBright Blue 700 Bio-Rad Cat #12004161; RRID:AB_2721073 Rabbit anti-BCL-XL Cell Signaling Technology Cat #2764T; RRID:AB_2228008 Rabbit anti-BIM Cell Signaling Technology Cat #2819S; RRID:AB_10692515 Rabbit anti-BAK Cell Signaling Technology Cat #3814S; RRID:AB_2290287 Rabbit anti-lipocalin-2 antibody Abcam Cat #ab63929; RRID:AB_1140965 Chicken anti-GFP Aves Labs Cat #GFP-1020; RRID:AB_10000240 Mouse anti-Beta III Tubulin Promega Cat #G7121; RRID:AB_430874 Rabbit anti-Phosphorylated Histone H3 Cell Signaling Technology Cat #4499; RRID:AB_10544537 Rabbit anti-Cleaved Caspase 3 Cell Signaling Technology Cat #9661; RRID:AB_2341188 Bacterial and virus strains psPAX2 (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12260; RRID:Addgene_12260 pMD2.G (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12259; RRID:Addgene_12259 MISSION pLKO.1-puro Non-Mammalian shRNA Control Sigma TRC #SHC002 Lcn2 shRNA KD1 Sigma TRC #TRCN0000055328 Lcn2 shRNA KD2 Sigma TRC #TRCN0000055331 Lcn2 shRNA KD3 Sigma TRC #TRCN0000055332 Biological samples Human glioblastoma patient tissue This paper, Cleveland Clinic See STAR Methods Human non-tumor patient tissue This paper, Cleveland Clinic See STAR Methods Primary mouse astrocytes Silver et al. 80 See STAR Methods Chemicals, peptides, and recombinant proteins RPMI 1640 medium Cleveland Clinic Media Preparation Core N/A Fetal bovine serum Cleveland Clinic Media Preparation Core N/A Penicillin-Streptomycin Cleveland Clinic Media Preparation Core N/A Trypsin-EDTA Cleveland Clinic Media Preparation Core N/A Trypan Blue ThermoFisher Cat #15250061 Image-It Lipid Peroxidation Kit Invitrogen Cat #C10445 CellRox Deep Red ThermoFisher Cat #C10422 Deferoxamine (DFO) Sigma Cat #D9533-1G RSL3 (GPX4 inhibitor) SelleckChem Cat #S8155 Erastin SelleckChem Cat #S7242 Ferrostatin-1 (Fer-1) SelleckChem Cat #S7243 Liproxstatin-1 (Lip-1) SelleckChem Cat #S7699 Necrostatin-1 SelleckChem Cat #ab141053 Z-VAD-FMK InvivoGen Cat #tlrl-vad PAC-1 SelleckChem Cat #S2738 Bemcentinib (R428, BGB324) SelleckChem Cat #S2841 1x NP-40 lysis buffer ThermoFisher Cat #J60766.AP Protease inhibitor cocktail Sigma-Aldrich Cat #P8340 (Continued on next page) 16 Cell Reports 45, 116965, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor cocktail Sigma-Aldrich Cat #P5725 PMSF Sigma-Aldrich Cat #10837091001 Sodium acetate ThermoFisher Cat #A13184-30 Thioglycolic acid Fisher Scientific Cat #M0052252G Chromogenic solution (FerroZine) MedChemExpress Cat #HY-137805 FITC-annexin V BioLegend Cat #640914 DRAQ7 ThermoFisher Cat #D15105 Critical commercial assays CellTiter Glo Cell Viability Assay Promega Cat #G7573 RNeasy Mini Kit Qiagen Cat #74106 qScript cDNA SuperMix Quanta Biosciences Cat #95048-100 Fast SYBR Green Mastermix ThermoFisher Cat #4385618 Proteome Profiler Mouse Phospho-RTK Array Kit R&D Systems Cat#ARY014 Experimental models: Cell lines OL61 (mouse glioma) Castro-Lowenstein Laboratory, Univ.
shRNA:Article Title: Lipocalin 2 orchestrates resistance to ferroptosis via AXL.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 Cell Signaling Technology Cat #52455S; RRID:AB_2924984 Rabbit anti-pAXL (Y702) ThermoFisher Cat #PA5-64862; RRID:AB_2662770 Rabbit anti-AXL (C terminus) LS Bio Cat # LS-B12012-100 hFAB TM Rhodamine Anti-Actin Bio-Rad Cat #120041163 Goat anti-rabbit StarBright Blue 700 Bio-Rad Cat #12004161; RRID:AB_2721073 Rabbit anti-BCL-XL Cell Signaling Technology Cat #2764T; RRID:AB_2228008 Rabbit anti-BIM Cell Signaling Technology Cat #2819S; RRID:AB_10692515 Rabbit anti-BAK Cell Signaling Technology Cat #3814S; RRID:AB_2290287 Rabbit anti-lipocalin-2 antibody Abcam Cat #ab63929; RRID:AB_1140965 Chicken anti-GFP Aves Labs Cat #GFP-1020; RRID:AB_10000240 Mouse anti-Beta III Tubulin Promega Cat #G7121; RRID:AB_430874 Rabbit anti-Phosphorylated Histone H3 Cell Signaling Technology Cat #4499; RRID:AB_10544537 Rabbit anti-Cleaved Caspase 3 Cell Signaling Technology Cat #9661; RRID:AB_2341188 Bacterial and virus strains psPAX2 (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12260; RRID:Addgene_12260 pMD2.G (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12259; RRID:Addgene_12259 MISSION pLKO.1-puro Non-Mammalian shRNA Control Sigma TRC #SHC002 Lcn2 shRNA KD1 Sigma TRC #TRCN0000055328 Lcn2 shRNA KD2 Sigma TRC #TRCN0000055331 Lcn2 shRNA KD3 Sigma TRC #TRCN0000055332 Biological samples Human glioblastoma patient tissue This paper, Cleveland Clinic See STAR Methods Human non-tumor patient tissue This paper, Cleveland Clinic See STAR Methods Primary mouse astrocytes Silver et al. 80 See STAR Methods Chemicals, peptides, and recombinant proteins RPMI 1640 medium Cleveland Clinic Media Preparation Core N/A Fetal bovine serum Cleveland Clinic Media Preparation Core N/A Penicillin-Streptomycin Cleveland Clinic Media Preparation Core N/A Trypsin-EDTA Cleveland Clinic Media Preparation Core N/A Trypan Blue ThermoFisher Cat #15250061 Image-It Lipid Peroxidation Kit Invitrogen Cat #C10445 CellRox Deep Red ThermoFisher Cat #C10422 Deferoxamine (DFO) Sigma Cat #D9533-1G RSL3 (GPX4 inhibitor) SelleckChem Cat #S8155 Erastin SelleckChem Cat #S7242 Ferrostatin-1 (Fer-1) SelleckChem Cat #S7243 Liproxstatin-1 (Lip-1) SelleckChem Cat #S7699 Necrostatin-1 SelleckChem Cat #ab141053 Z-VAD-FMK InvivoGen Cat #tlrl-vad PAC-1 SelleckChem Cat #S2738 Bemcentinib (R428, BGB324) SelleckChem Cat #S2841 1x NP-40 lysis buffer ThermoFisher Cat #J60766.AP Protease inhibitor cocktail Sigma-Aldrich Cat #P8340 (Continued on next page) 16 Cell Reports 45, 116965, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor cocktail Sigma-Aldrich Cat #P5725 PMSF Sigma-Aldrich Cat #10837091001 Sodium acetate ThermoFisher Cat #A13184-30 Thioglycolic acid Fisher Scientific Cat #M0052252G Chromogenic solution (FerroZine) MedChemExpress Cat #HY-137805 FITC-annexin V BioLegend Cat #640914 DRAQ7 ThermoFisher Cat #D15105 Critical commercial assays CellTiter Glo Cell Viability Assay Promega Cat #G7573 RNeasy Mini Kit Qiagen Cat #74106 qScript cDNA SuperMix Quanta Biosciences Cat #95048-100 Fast SYBR Green Mastermix ThermoFisher Cat #4385618 Proteome Profiler Mouse Phospho-RTK Array Kit R&D Systems Cat#ARY014 Experimental models: Cell lines OL61 (mouse glioma) Castro-Lowenstein Laboratory, Univ.
Control:Article Title: Lipocalin 2 orchestrates resistance to ferroptosis via AXL.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 Cell Signaling Technology Cat #52455S; RRID:AB_2924984 Rabbit anti-pAXL (Y702) ThermoFisher Cat #PA5-64862; RRID:AB_2662770 Rabbit anti-AXL (C terminus) LS Bio Cat # LS-B12012-100 hFAB TM Rhodamine Anti-Actin Bio-Rad Cat #120041163 Goat anti-rabbit StarBright Blue 700 Bio-Rad Cat #12004161; RRID:AB_2721073 Rabbit anti-BCL-XL Cell Signaling Technology Cat #2764T; RRID:AB_2228008 Rabbit anti-BIM Cell Signaling Technology Cat #2819S; RRID:AB_10692515 Rabbit anti-BAK Cell Signaling Technology Cat #3814S; RRID:AB_2290287 Rabbit anti-lipocalin-2 antibody Abcam Cat #ab63929; RRID:AB_1140965 Chicken anti-GFP Aves Labs Cat #GFP-1020; RRID:AB_10000240 Mouse anti-Beta III Tubulin Promega Cat #G7121; RRID:AB_430874 Rabbit anti-Phosphorylated Histone H3 Cell Signaling Technology Cat #4499; RRID:AB_10544537 Rabbit anti-Cleaved Caspase 3 Cell Signaling Technology Cat #9661; RRID:AB_2341188 Bacterial and virus strains psPAX2 (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12260; RRID:Addgene_12260 pMD2.G (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12259; RRID:Addgene_12259 MISSION pLKO.1-puro Non-Mammalian shRNA Control Sigma TRC #SHC002 Lcn2 shRNA KD1 Sigma TRC #TRCN0000055328 Lcn2 shRNA KD2 Sigma TRC #TRCN0000055331 Lcn2 shRNA KD3 Sigma TRC #TRCN0000055332 Biological samples Human glioblastoma patient tissue This paper, Cleveland Clinic See STAR Methods Human non-tumor patient tissue This paper, Cleveland Clinic See STAR Methods Primary mouse astrocytes Silver et al. 80 See STAR Methods Chemicals, peptides, and recombinant proteins RPMI 1640 medium Cleveland Clinic Media Preparation Core N/A Fetal bovine serum Cleveland Clinic Media Preparation Core N/A Penicillin-Streptomycin Cleveland Clinic Media Preparation Core N/A Trypsin-EDTA Cleveland Clinic Media Preparation Core N/A Trypan Blue ThermoFisher Cat #15250061 Image-It Lipid Peroxidation Kit Invitrogen Cat #C10445 CellRox Deep Red ThermoFisher Cat #C10422 Deferoxamine (DFO) Sigma Cat #D9533-1G RSL3 (GPX4 inhibitor) SelleckChem Cat #S8155 Erastin SelleckChem Cat #S7242 Ferrostatin-1 (Fer-1) SelleckChem Cat #S7243 Liproxstatin-1 (Lip-1) SelleckChem Cat #S7699 Necrostatin-1 SelleckChem Cat #ab141053 Z-VAD-FMK InvivoGen Cat #tlrl-vad PAC-1 SelleckChem Cat #S2738 Bemcentinib (R428, BGB324) SelleckChem Cat #S2841 1x NP-40 lysis buffer ThermoFisher Cat #J60766.AP Protease inhibitor cocktail Sigma-Aldrich Cat #P8340 (Continued on next page) 16 Cell Reports 45, 116965, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor cocktail Sigma-Aldrich Cat #P5725 PMSF Sigma-Aldrich Cat #10837091001 Sodium acetate ThermoFisher Cat #A13184-30 Thioglycolic acid Fisher Scientific Cat #M0052252G Chromogenic solution (FerroZine) MedChemExpress Cat #HY-137805 FITC-annexin V BioLegend Cat #640914 DRAQ7 ThermoFisher Cat #D15105 Critical commercial assays CellTiter Glo Cell Viability Assay Promega Cat #G7573 RNeasy Mini Kit Qiagen Cat #74106 qScript cDNA SuperMix Quanta Biosciences Cat #95048-100 Fast SYBR Green Mastermix ThermoFisher Cat #4385618 Proteome Profiler Mouse Phospho-RTK Array Kit R&D Systems Cat#ARY014 Experimental models: Cell lines OL61 (mouse glioma) Castro-Lowenstein Laboratory, Univ.
Recombinant:Article Title: Lipocalin 2 orchestrates resistance to ferroptosis via AXL.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 Cell Signaling Technology Cat #52455S; RRID:AB_2924984 Rabbit anti-pAXL (Y702) ThermoFisher Cat #PA5-64862; RRID:AB_2662770 Rabbit anti-AXL (C terminus) LS Bio Cat # LS-B12012-100 hFAB TM Rhodamine Anti-Actin Bio-Rad Cat #120041163 Goat anti-rabbit StarBright Blue 700 Bio-Rad Cat #12004161; RRID:AB_2721073 Rabbit anti-BCL-XL Cell Signaling Technology Cat #2764T; RRID:AB_2228008 Rabbit anti-BIM Cell Signaling Technology Cat #2819S; RRID:AB_10692515 Rabbit anti-BAK Cell Signaling Technology Cat #3814S; RRID:AB_2290287 Rabbit anti-lipocalin-2 antibody Abcam Cat #ab63929; RRID:AB_1140965 Chicken anti-GFP Aves Labs Cat #GFP-1020; RRID:AB_10000240 Mouse anti-Beta III Tubulin Promega Cat #G7121; RRID:AB_430874 Rabbit anti-Phosphorylated Histone H3 Cell Signaling Technology Cat #4499; RRID:AB_10544537 Rabbit anti-Cleaved Caspase 3 Cell Signaling Technology Cat #9661; RRID:AB_2341188 Bacterial and virus strains psPAX2 (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12260; RRID:Addgene_12260 pMD2.G (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12259; RRID:Addgene_12259 MISSION pLKO.1-puro Non-Mammalian shRNA Control Sigma TRC #SHC002 Lcn2 shRNA KD1 Sigma TRC #TRCN0000055328 Lcn2 shRNA KD2 Sigma TRC #TRCN0000055331 Lcn2 shRNA KD3 Sigma TRC #TRCN0000055332 Biological samples Human glioblastoma patient tissue This paper, Cleveland Clinic See STAR Methods Human non-tumor patient tissue This paper, Cleveland Clinic See STAR Methods Primary mouse astrocytes Silver et al. 80 See STAR Methods Chemicals, peptides, and recombinant proteins RPMI 1640 medium Cleveland Clinic Media Preparation Core N/A Fetal bovine serum Cleveland Clinic Media Preparation Core N/A Penicillin-Streptomycin Cleveland Clinic Media Preparation Core N/A Trypsin-EDTA Cleveland Clinic Media Preparation Core N/A Trypan Blue ThermoFisher Cat #15250061 Image-It Lipid Peroxidation Kit Invitrogen Cat #C10445 CellRox Deep Red ThermoFisher Cat #C10422 Deferoxamine (DFO) Sigma Cat #D9533-1G RSL3 (GPX4 inhibitor) SelleckChem Cat #S8155 Erastin SelleckChem Cat #S7242 Ferrostatin-1 (Fer-1) SelleckChem Cat #S7243 Liproxstatin-1 (Lip-1) SelleckChem Cat #S7699 Necrostatin-1 SelleckChem Cat #ab141053 Z-VAD-FMK InvivoGen Cat #tlrl-vad PAC-1 SelleckChem Cat #S2738 Bemcentinib (R428, BGB324) SelleckChem Cat #S2841 1x NP-40 lysis buffer ThermoFisher Cat #J60766.AP Protease inhibitor cocktail Sigma-Aldrich Cat #P8340 (Continued on next page) 16 Cell Reports 45, 116965, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor cocktail Sigma-Aldrich Cat #P5725 PMSF Sigma-Aldrich Cat #10837091001 Sodium acetate ThermoFisher Cat #A13184-30 Thioglycolic acid Fisher Scientific Cat #M0052252G Chromogenic solution (FerroZine) MedChemExpress Cat #HY-137805 FITC-annexin V BioLegend Cat #640914 DRAQ7 ThermoFisher Cat #D15105 Critical commercial assays CellTiter Glo Cell Viability Assay Promega Cat #G7573 RNeasy Mini Kit Qiagen Cat #74106 qScript cDNA SuperMix Quanta Biosciences Cat #95048-100 Fast SYBR Green Mastermix ThermoFisher Cat #4385618 Proteome Profiler Mouse Phospho-RTK Array Kit R&D Systems Cat#ARY014 Experimental models: Cell lines OL61 (mouse glioma) Castro-Lowenstein Laboratory, Univ.
Lysis:Article Title: Lipocalin 2 orchestrates resistance to ferroptosis via AXL.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 Cell Signaling Technology Cat #52455S; RRID:AB_2924984 Rabbit anti-pAXL (Y702) ThermoFisher Cat #PA5-64862; RRID:AB_2662770 Rabbit anti-AXL (C terminus) LS Bio Cat # LS-B12012-100 hFAB TM Rhodamine Anti-Actin Bio-Rad Cat #120041163 Goat anti-rabbit StarBright Blue 700 Bio-Rad Cat #12004161; RRID:AB_2721073 Rabbit anti-BCL-XL Cell Signaling Technology Cat #2764T; RRID:AB_2228008 Rabbit anti-BIM Cell Signaling Technology Cat #2819S; RRID:AB_10692515 Rabbit anti-BAK Cell Signaling Technology Cat #3814S; RRID:AB_2290287 Rabbit anti-lipocalin-2 antibody Abcam Cat #ab63929; RRID:AB_1140965 Chicken anti-GFP Aves Labs Cat #GFP-1020; RRID:AB_10000240 Mouse anti-Beta III Tubulin Promega Cat #G7121; RRID:AB_430874 Rabbit anti-Phosphorylated Histone H3 Cell Signaling Technology Cat #4499; RRID:AB_10544537 Rabbit anti-Cleaved Caspase 3 Cell Signaling Technology Cat #9661; RRID:AB_2341188 Bacterial and virus strains psPAX2 (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12260; RRID:Addgene_12260 pMD2.G (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12259; RRID:Addgene_12259 MISSION pLKO.1-puro Non-Mammalian shRNA Control Sigma TRC #SHC002 Lcn2 shRNA KD1 Sigma TRC #TRCN0000055328 Lcn2 shRNA KD2 Sigma TRC #TRCN0000055331 Lcn2 shRNA KD3 Sigma TRC #TRCN0000055332 Biological samples Human glioblastoma patient tissue This paper, Cleveland Clinic See STAR Methods Human non-tumor patient tissue This paper, Cleveland Clinic See STAR Methods Primary mouse astrocytes Silver et al. 80 See STAR Methods Chemicals, peptides, and recombinant proteins RPMI 1640 medium Cleveland Clinic Media Preparation Core N/A Fetal bovine serum Cleveland Clinic Media Preparation Core N/A Penicillin-Streptomycin Cleveland Clinic Media Preparation Core N/A Trypsin-EDTA Cleveland Clinic Media Preparation Core N/A Trypan Blue ThermoFisher Cat #15250061 Image-It Lipid Peroxidation Kit Invitrogen Cat #C10445 CellRox Deep Red ThermoFisher Cat #C10422 Deferoxamine (DFO) Sigma Cat #D9533-1G RSL3 (GPX4 inhibitor) SelleckChem Cat #S8155 Erastin SelleckChem Cat #S7242 Ferrostatin-1 (Fer-1) SelleckChem Cat #S7243 Liproxstatin-1 (Lip-1) SelleckChem Cat #S7699 Necrostatin-1 SelleckChem Cat #ab141053 Z-VAD-FMK InvivoGen Cat #tlrl-vad PAC-1 SelleckChem Cat #S2738 Bemcentinib (R428, BGB324) SelleckChem Cat #S2841 1x NP-40 lysis buffer ThermoFisher Cat #J60766.AP Protease inhibitor cocktail Sigma-Aldrich Cat #P8340 (Continued on next page) 16 Cell Reports 45, 116965, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor cocktail Sigma-Aldrich Cat #P5725 PMSF Sigma-Aldrich Cat #10837091001 Sodium acetate ThermoFisher Cat #A13184-30 Thioglycolic acid Fisher Scientific Cat #M0052252G Chromogenic solution (FerroZine) MedChemExpress Cat #HY-137805 FITC-annexin V BioLegend Cat #640914 DRAQ7 ThermoFisher Cat #D15105 Critical commercial assays CellTiter Glo Cell Viability Assay Promega Cat #G7573 RNeasy Mini Kit Qiagen Cat #74106 qScript cDNA SuperMix Quanta Biosciences Cat #95048-100 Fast SYBR Green Mastermix ThermoFisher Cat #4385618 Proteome Profiler Mouse Phospho-RTK Array Kit R&D Systems Cat#ARY014 Experimental models: Cell lines OL61 (mouse glioma) Castro-Lowenstein Laboratory, Univ.
Protease Inhibitor:Article Title: Lipocalin 2 orchestrates resistance to ferroptosis via AXL.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 Cell Signaling Technology Cat #52455S; RRID:AB_2924984 Rabbit anti-pAXL (Y702) ThermoFisher Cat #PA5-64862; RRID:AB_2662770 Rabbit anti-AXL (C terminus) LS Bio Cat # LS-B12012-100 hFAB TM Rhodamine Anti-Actin Bio-Rad Cat #120041163 Goat anti-rabbit StarBright Blue 700 Bio-Rad Cat #12004161; RRID:AB_2721073 Rabbit anti-BCL-XL Cell Signaling Technology Cat #2764T; RRID:AB_2228008 Rabbit anti-BIM Cell Signaling Technology Cat #2819S; RRID:AB_10692515 Rabbit anti-BAK Cell Signaling Technology Cat #3814S; RRID:AB_2290287 Rabbit anti-lipocalin-2 antibody Abcam Cat #ab63929; RRID:AB_1140965 Chicken anti-GFP Aves Labs Cat #GFP-1020; RRID:AB_10000240 Mouse anti-Beta III Tubulin Promega Cat #G7121; RRID:AB_430874 Rabbit anti-Phosphorylated Histone H3 Cell Signaling Technology Cat #4499; RRID:AB_10544537 Rabbit anti-Cleaved Caspase 3 Cell Signaling Technology Cat #9661; RRID:AB_2341188 Bacterial and virus strains psPAX2 (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12260; RRID:Addgene_12260 pMD2.G (lentiviral packaging plasmid) Addgene (Didier Trono) Cat #12259; RRID:Addgene_12259 MISSION pLKO.1-puro Non-Mammalian shRNA Control Sigma TRC #SHC002 Lcn2 shRNA KD1 Sigma TRC #TRCN0000055328 Lcn2 shRNA KD2 Sigma TRC #TRCN0000055331 Lcn2 shRNA KD3 Sigma TRC #TRCN0000055332 Biological samples Human glioblastoma patient tissue This paper, Cleveland Clinic See STAR Methods Human non-tumor patient tissue This paper, Cleveland Clinic See STAR Methods Primary mouse astrocytes Silver et al. 80 See STAR Methods Chemicals, peptides, and recombinant proteins RPMI 1640 medium Cleveland Clinic Media Preparation Core N/A Fetal bovine serum Cleveland Clinic Media Preparation Core N/A Penicillin-Streptomycin Cleveland Clinic Media Preparation Core N/A Trypsin-EDTA Cleveland Clinic Media Preparation Core N/A Trypan Blue ThermoFisher Cat #15250061 Image-It Lipid Peroxidation Kit Invitrogen Cat #C10445 CellRox Deep Red ThermoFisher Cat #C10422 Deferoxamine (DFO) Sigma Cat #D9533-1G RSL3 (GPX4 inhibitor) SelleckChem Cat #S8155 Erastin SelleckChem Cat #S7242 Ferrostatin-1 (Fer-1) SelleckChem Cat #S7243 Liproxstatin-1 (Lip-1) SelleckChem Cat #S7699 Necrostatin-1 SelleckChem Cat #ab141053 Z-VAD-FMK InvivoGen Cat #tlrl-vad PAC-1 SelleckChem Cat #S2738 Bemcentinib (R428, BGB324) SelleckChem Cat #S2841 1x NP-40 lysis buffer ThermoFisher Cat #J60766.AP Protease inhibitor cocktail Sigma-Aldrich Cat #P8340 (Continued on next page) 16 Cell Reports 45, 116965, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase inhibitor cocktail Sigma-Aldrich Cat #P5725 PMSF Sigma-Aldrich Cat #10837091001 Sodium acetate ThermoFisher Cat #A13184-30 Thioglycolic acid Fisher Scientific Cat #M0052252G Chromogenic solution (FerroZine) MedChemExpress Cat #HY-137805 FITC-annexin V BioLegend Cat #640914 DRAQ7 ThermoFisher Cat #D15105 Critical commercial assays CellTiter Glo Cell Viability Assay Promega Cat #G7573 RNeasy Mini Kit Qiagen Cat #74106 qScript cDNA SuperMix Quanta Biosciences Cat #95048-100 Fast SYBR Green Mastermix ThermoFisher Cat #4385618 Proteome Profiler Mouse Phospho-RTK Array Kit R&D Systems Cat#ARY014 Experimental models: Cell lines OL61 (mouse glioma) Castro-Lowenstein Laboratory, Univ.
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